MONITORING AND DISTRIBUTION OF TETRACYCLINES RESIDUE IN BONE AND SOFT TISSUES OF BROILERS

The presence of certain antimicrobial agents in meat and other animal products constitute a potential hazard for the consumer and may cause allergic reactions, interference in the intestinal flora, and resistant population of bacteria in the general population, thereby rendering antibiotic treatment ineffective. The present work was conducted to study the correlation between the distribution in bone and soft tissue of tetracycline fluorescence examined under UV-light microscopy and withdrawal period. Semi quantitative histomorphometric analysis of soft tissue and tibia of broilers were performed after tetracycline labeling. For this purpose, 15 broilers approximately 1 kg in weight were subdivided into two groups one experimental group ( 10 treated birds ) and One control group (5 control untreated birds ). Each bird of the experimental group was given intraperitoneally 10 mg of tetracycline and slaughtered after 1, 3, 6, 9 and 12 days of the drug a ministration. Frozen sections were made from kidneys and liver. Tibias from both sides we collected; one tibia was fixed in 10 % formal solution, while the other was left unfixed. Both tibias were used for preparation of undecalcified materials. The later consisted of sagittal section of the metaphyseal plate and cross sections of diaphysis. All soft tissue and bone sections were examined under the fluorescence microscope Using image analysis, the thickness of both proliferating and hypertrophying cartilage layers the front fluorescent line of the metaphysis ; the osteoid seams lining the trabeculae in spongious layer were measured and correl with the duration of withdrawal.