TRIALS FOR PREPARATION OF A COMBINED INACTIVATED VACCINE OF SALMONELLOSIS AND FOWEL CHOLERA IN POULTRY

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Veterinary serum and vaccine Research Institute, Abbasia, Cairo, Egypt.

Abstract

In an attempt for preparation a combined inactivated vaccine containing P. multocida, S.typhimurium and S. enteritidis, this vacine was injected in a dose of 0.5ml S/C twice one month apart, in groups of chickens. The humoral immunity was measured by  indirect haemagelutination (IHA) and enzyme linked immunosobent assay (ELISA) test. The combined vaccine elicited high levels of antibody and Showed protection rate of 96.5% against virulent challenge with these bacteria. The cellular immune response was measured by 3-(4,5 - dimethyl- thiazol - 2- yl) 2,5- diphenyl tetrazolium bromide  (MTT) utilization test and heterophil /lymphocyte ratio. the conjugation of these organisms conferred T-dependent properties on their lipopolysaccharides. The combination had no mutual competitive effect but enchancing each other including improvement the immunogenicity of fowl cholera vaccine. The combined vaccine seems to be a good economical value for poultry industry with less shedding of salmonellae after challenge with virulent strain.

Main Subjects

Veterinary Medical Journal (Giza)
Volume 46, Issue 4
October 1998
Pages 867-875

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