STUDIES ON THE STABILITY OF RVF VIRUS

Authors

Veterinary Serum and Vaccine Research Institute Abbasia . Cairo.

Abstract

RIFT Valley Fever virus is one of the weak viruses. So this study was made to know the degree of stability of the virus at different temperatures, how to deal with it and how to keep it for a long time without great loss in its titre by using different stabilizers at different temperatures especially at the diagnostic and vaccine production Laboratories. In this study , we titrated a lot of the virus and it was 8.5 log 10 TCID 50 /mL. We divided it into groups and kept at 37°C, _ 4°C, 20°C and -70'C without using any stabilizer then another groups were Kept at -20°C and - 70°C using different percentages of peptone with sucrose, lact albumin with sucrose , and skimmed milk . It was found that the titre of the virus decreased about 2 log 10 TCID50 /ml after 2 months at  -20°C but using Skimmed milk, the loss in the virus titre after 12 months was not so great . While at  -70 c ,the virus about 2 log10 TCID50 /ml. after 6 month of Preservation . It was found that 37°C is not suitable to keep the virus for not more than 2 hours as its titer was 5.8 log10 TCID50 /ml while at 4 'C the Virus was still suitable for use up till 3 days as its titer was 6.1 log 10 TCID50 /ml From this study , it was recommend to use 3% skimmed milk as a stabilizer to preserve the virus at - 20 ‘C, for 6 months and for twelve months at -70 ‘C, without great loss in its titre . Also it was found that 37 C, is not suitable to keep the virus more than 2 hours although the virus was sill present but with very low titre (0.7 log 10TCID50 /ml ) after seven days of Preservation.

Main Subjects

Veterinary Medical Journal (Giza)
Volume 46, Issue 4
October 1998
Pages 729-735

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