ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA) FOR DETECTION OF BVD VIRUS ANTIGEN IN BUFFY COATS OF MUCOSAL DISEASE AFFECTED CATTLE

Authors

1 Members of Animal Medicine Department, Assiut University, Assiut, Egypt.

2 Professor of Internal Medicine & Director Ruminants Clinic, Justus- Liebig University, Giessen ,Germany

Abstract

Enzyme linked immunosorbent assay (ELISA) in comparison with tissue culture method was used for detection of bovine viral diarrhea virus (BVDH) antigen in 70 out 152 buffy coats from Mucosal disease affected cattle which were submitted to cattle clinic in Justus Liebig University, Giessen, Germany from 1994 to 1996. ELSA and tissue culture methods were performed in the Institute of Animal Hygiene and infectious Diseases, Justus Liebig University, Giessen, Germany. The results showed that, 58 (82.9%) and 61 (87.1) of buffy coats were positive in cell culture and direct ELISA respectively. All samples which were positive in cell culture were also positive in ELISA. Three cattle with chronic mucosal disease were positive with direct ELISA, but negative with cell culture method. In conclusion, diagnosis of mucosal disease by isolation and detection of BVD virus using tissue culture method is a time consuming technique. Direct ELISA is a sensitive, rapid, but expensive method for the detection of BVD virus antigen in comparison to the cell technique.

Main Subjects

Veterinary Medical Journal (Giza)
Volume 46, Issue 4
October 1998
Pages 701-708

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