Comparison of two different extenders for cryopreservation of epididymal dog spermatozoa

Authors

1 Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University

2 Animal Reproduction Research Institute, Giza

Abstract

Post-mortem recovery and cryopreservation of viable sperm from the epididymis of genetically valuable animals or endangered species is an important technique for preserving male gametes and thus for maintaining a germ-plasm bank. The extender type used to freeze sperm is important to avoid spermatozoal membrane damage and to preserve semen quality after cryopreservation. The objective of this study was to determine the effects of commercial bovine extender (BullXcell®, IMV, L’Aigle, France) and Tris-citric acid -fructose egg yolk 7% glycerol extender (TCF) on cryopreservation of canine epididymal sperm. The testes of ten adult Egyptian domestic dogs were kept at 5°C in a saline solution and transported to the laboratory within 24 h. Diluted samples were cooled slowly to 5°C over 2 h and equilibrated at that temperature for 2h. Aliquots of samples were loaded into 0.25 ml straws and frozen in liquid nitrogen vapor for 10 minutes and stored in liquid nitrogen. The semen parameters: Post-thaw progressive motility, viability index, membrane integrity (HOST), and acrosomal integrity (sliver nitrate Stain) were evaluated, Results indicated that membrane integrity (HOST) and intact acrosome were statistically higher in BullX, extender. We concluded that BullXcell® is a suitable alternative for the freezing of canine epididymal sperm. 

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