COMPARATIVE PATHOLOGICAL AND IMMUNOLOGICAL STUDIES ON DIFFERENT OIL ADJUVANATED INACTIVATED NEWCASTLE DISEASE VIRUS VACCINES (NDVV) PRIVATE

ABD EL-HADY, AMAL; EL-EBIARY, ELHAM; OSMAN, A; HASSAN, NADIA

doi:10.21608/vmjg.2002.369353

COMPARATIVE PATHOLOGICAL AND IMMUNOLOGICAL STUDIES ON DIFFERENT OIL ADJUVANATED INACTIVATED NEWCASTLE DISEASE VIRUS VACCINES (NDVV) PRIVATE

Document Type : Original Article

Authors

¹ Central Laboratory for Control of Veterinary Biologics, Abbasia, Cairo.

² Department of Pathology, Faculty of Veterinary Medicine, Cairo University

³ Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo.

10.21608/vmjg.2002.369353

Abstract

The present study was applied to evaluate the physical properties of ISA-70 Marcol-52 and paraffin oil adjuvant used in commercial vaccines and describes the pathological changes and serological response of chickens after intramuscular injection. A total of 140 three-week old white Leghorn chickens, specific pathogen free were obtained from Koum Osheim, Fayoum Governorate. Chickens were divided into four equal groups one of them is control, the other three groups were in- jected intramuscularly with the following oil adjuvant emulsions containing inactivated Newcastle disease virus (NDV) antigen: ISA-70, Marcol-52 And paraffin. Five chickens of each group were Sacrificed at 72 hours and at 1, 2, 4, 8, 12 and 16 weeks post-vaccination.
The pathological reaction induced by ISA-70 emulsion was characterized by focal aggregation of macrophages, lymphocytes, epithelioid cells and fibroblasts around the small cysts in muscle. These lesions peaked at 2 weeks post-injection and considered as mild pathological changes if compared with Marcol-52 and paraffin oil adjuVant. The serological findings revealed that the enhancing effect of ISA-70 on NDV antigen was more than that of Marcol-52 and paraffin oil adjuVant.

Main Subjects