Beneficial Effects of Pinocembrin and Quercetin on the general conditions, immune parameters and gut lactobacilli in broiler chickens

Bastamy, Mustafa; Elhady, Mohamed; Raheel, Ismail; Elbestawy, Ahmed; Abdel-Latif, Mervat; Orabi, Ahmed

doi:10.21608/vmjg.2025.401082.1048

Beneficial Effects of Pinocembrin and Quercetin on the general conditions, immune parameters and gut lactobacilli in broiler chickens

Document Type : Original Article

Authors

¹ Department of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University, Egypt

² Department of Toxicology and Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Egypt

³ Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni Suef University, Egypt

⁴ Department of Bird and Rabbit Diseases, Faculty of Veterinary Medicine, Menoufia University, Egypt

⁵ Department of Nutrition and Veterinary Clinical Nutrition, Faculty of Veterinary Medicine, Damanhour University, Egypt

⁶ Department of Microbiology, Faculty of Veterinary Medicine, Cairo University

10.21608/vmjg.2025.401082.1048

Abstract

There are various stressors that may contribute to commercial poultry production leading to economic losses due to decreased productivity and performance of growing chickens. Understanding the molecular pathways during the oxidative stress in birds is critical in the current immunological research. In this study, pinocembrin and quercetin are examined to explore their anti-inflammatory, antioxidant, hepatoprotective effects in broiler chickens along with their impact on gut health, and meat quality. A total of 300 one day old broiler chicks (Ross 308) were randomly allocated and divided into 2 groups. G1was kept as control non-treated and G2 received 2 supplements of Liba-Ton^® as a pinocembrin natural source from propolis and Radical-Free^®, a synthetic quercetin, for 8 hrs. daily for 3 days each week until the end of experiment at 35 days old. Parameters of growth (BWG, FI, and FCR), immune organ index (spleen, thymus, and bursa), anti-inflammatory signaling (IL-B1 and Phagocytic index), liver biochemistry (AST, ALT, AKP, and GT), antioxidant activity (SOD, CAT, TAC, and GSH in liver and muscle tissues) were assayed. The obtained results revealed positive impact of pinocembrin and quercetin supplementation in broilers. Carcass yield and muscle quality revealed that the dressing percentage was significantly increased in G2 to 73.63% as compared to 73.3% in G1. Also, the giblet percentage revealed significant increase of liver, heart, gizzard (2.35, 0.54 and 2.17 respectively), while breast muscle yield showed significant increase from 38.2% in chickens of G1 to 41.6% in G2 with 5.8 pH, 24hr and 71.55 water holding capacity.

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