Effect of combined mesenchymal stem cell and derived exosomes in control of STZ induced diabetic hepatopathy in Wistar rat model

Document Type : Original Article

Authors

1 Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

2 Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

3 Department of Toxicology and Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

Abstract

Diabetes mellitus (DM) is a chronic widespread metabolic disorder, involving a high blood glucose level which causes overtime multiple serious complications. Due to the unwanted side effects of the anti-diabetic medicines and high dose required of the herbal plants which used for a long time for DM treatment. The current studies directed to the use stem cells and exosomes as regenerative medicine to overcome these limitations of traditional therapy.
We aimed to investigate the antidiabetic effect of combined Bone marrow mesenchymal stem cells and exosomes against Diabetes induced by streptozotocin (STZ) in Wistar rats through hindering the inflammatory reactions and the hypolipidemic effect by preventing lipid accumulation.
Our study was conducted on 21 male Wistar rats divided into three groups (control non diabetic group, control diabetic group and combined Stem cell and exosomes treated group). Blood glucose level and liver function enzymes were assayed in addition to a lipid profile test. Moreover, the enzymatic and non-enzymatic antioxidants were assayed in addition to lipid peroxidation products in the liver. It was found that the injection combination of both stem cells and exosomes into diabetic rats improved the destructive effects that happened as a result of STZ injection and reinstated the biochemical functions in addition to levels of hepatic antioxidants to normal.

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