STUDIES OF BOVINE FIELD SKIN LESIONS IN FAYOUM GOVERNORATE

Authors

1 Department of Virology, Animal Health Research institute, Dokki, Giza.

2 Department of Animal Health Research institute. Dokki, Giza.

Abstract

In this study an outbreak in Fayoum Governorate during summer 1998 showed skin lesions, fever (40-41℃) which resembled clinical manifestation of lumpy skin disease (LSD). The skin lesions in a form  of firm rounded skin nodules which covered with erected hair. 15 cases among cattle hed have been recorded, samples from all suspected diseased animals were taken. The samples were inoculated on chorio-allantoic membrane (CAM) of emberyonated chickenegg (ECE) aged 9-11 days, for several passage and in MBDK tussue culture for isolation. Confirmatory tests were carries out by using standard specific antisera against LSD virus using agar gel immunodiffusion test AGID, immunoperoxidase and immunofluorescent. The positive lesion of CAM (pock lesion) were collected in 10% neutral formalin buffer for histopathological examination.

Main Subjects