TRIAL FOR PREPARATION OF COMBINED OIL INACTIVATED VACCINE AGAINST NEWCASTLE AND FOWL CHOLERA DISEASES BY USING MONTANIDE ISA206 OIL FOR IMPROVING THE IMMUNE RESPONSE IN CHICKENS

YOUSSEF, ELHAM A; HASSAN, EMAN A; EL-KOFFY, MERVAT A; DAOUD, A

doi:10.21608/vmjg.2005.384707

TRIAL FOR PREPARATION OF COMBINED OIL INACTIVATED VACCINE AGAINST NEWCASTLE AND FOWL CHOLERA DISEASES BY USING MONTANIDE ISA206 OIL FOR IMPROVING THE IMMUNE RESPONSE IN CHICKENS

Document Type : Original Article

Authors

Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo

10.21608/vmjg.2005.384707

Abstract

One hundred and fifty chickens were divided into 3 equal groups (50 birds each). Groups one and two were vaccinated with combined inactivated fowl cholera (FC) and Newcastle (ND) vaccines using ISA206 and white oil (paraffin oil) as adjuvants respectively while group three was kept as non-vaccinated control group. The results of haemagglutinating antibody titres against NDV revealed that ISA206 gave earlier and higher immune response than white oil till 8 weeks post vaccination (WPV) but from 16 till 20 WPV it Was noticed that paraffin oil was protective than ISA206. On the other hand, results of indirect haemagglutinating (IHA) and ELISA tests indicated that the same above results till the 6 WPV for FC by using ISA206, meanwhile paraffin oil recorded higher antibody titre from 8 WPV till the end of experiment and these results were confirmed by protection tests against virulent NDV and virulent FC.

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