VIROLOGICAL AND PATHOLOGICAL STUDIES ON BUFFALO POX VIRUS ISOLATED FROM DAIRY BUFFALOES UDDER

Document Type : Original Article

Authors

1 Pathology of Reproduction Department, Animal Reproduction Research Institute

2 Virology Department, Animal Health Research Institute

Abstract

Pox like lesions were frequently recorded in 43.75% dairy buffaloes recently introduced into small and moderate dairy barns at Kafr EL-Gabal and Zawet Abo Mesalem districts at Giza Governorate. These affections led to reduction of milk yield. This work was done to isolate and identify the causative agent to overcome the problem and its economic losses. After inoculation of the prepared collected sam­ples on the chorioallantoic membrane of embryo­nated chicken eggs, pock lesions were observed on the 4th or 5th day-post inoculation. Impression smears and samples were prepared from each har­vested chorioallantoic membrane for identifica­tion of the pox virus using Agar gel precipitation and indirect immunofluorescent techniques. Both tests showed positive results in all examined sam­ples in the form of precipitin lines or specific cy­toplasmic fluorescent reaction, respectively. Gross examination of the infected udders primari­ly revealed, tenderness followed by embedded or superficial papule with eroded or ulcerated sur­faces on the teats and lower, part of the udder. Histopathological study revealed focal infiltration of mononuclear and polymorphnuclear leucocy­tes in the dermal papillae with exocytosis of the inflammatory cells. Mild oedema and perivascu­lar cuffing were also seen in the dermal layer. The cells of the stratum spinosum showed bal­looning in addition to necrobiotc changes particu­larly near the ulcerative areas that was highly in­filtrated by polymorphnuclear leucocytes.

Main Subjects

Veterinary Medical Journal (Giza)
Volume 53, Issue 2
April 2005
Pages 267-278

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