IMPORTANCE OF BACTERIOCIN OF LACTOBACILLUS ACIDOPHILUS IN PREVENTION , COLONIZATION AND PROLIFERATION OF ENTERIC BACTERIA IN CATTLE

Document Type : Original Article

Authors

Bacteriology Department Animal Health Research Institute Dokki, Cairo, Egypt

Abstract

Bacteriocin produced by Lactobacillus acidophilus was tested for it's potential as aprobiotic cullure, it's exhibited good sensitivity to heat, pro- tease enzyme, acid tolerance, as well as bile resistance in media containing 0.3% bile acids. Bacteriocin was active over wide pH range and inhibited a number of pathogenic bacteria. The SDS - PAGE of the active fraction resulted in a single band with molecular mass of 5.4 KDa. In challenge test. Mice that were fed on milk based diets contain bacteriocin (108 C.F.U).showed 100%. protection level . Meanwhile the antibody titers in the serum of immunized mice with bacteriocin were significantly higher than the titers in the control of unprotected. mice . These results confirmed the probiotic effect of bacteriocin against colonization of most microorganism in the animal tissue as well as enhancing their immune response.

Main Subjects