INCIDENCE AND SDS -PAGE PROTEIN PROFILE ANALYSIS FOR MYCOPLASMA SPP, AND ACHOLEPLASMA SPP. ISOLATED FROM CAMELS IN EGYPT

Document Type : Original Article

Authors

1 Department of Microbiology, Faculty of Veterinary Medicine, Cairo University.

2 Animal Health Research Institute, Dokki ,Cairo

Abstract

Thirty-nine strains of Mycoplasma spp. (33) and Acholeplasma .spp. (6) were isolated from 118 pneumonic lungs and 110 tracheal samples ob­tained from different abattoirs in Egypt. The per­centages of their isolaion were 8.3 % from pneu­monic lungs and 8.7 % from tracheal samples. Based on the biochemical profile and growth inhi­bition results, the camel isolates were identified as, M. hovis, glucose positive mycoplasma and arginini. The identity of these isolates was fur­ther confirmed by growth inhibition test using a panel of specific antisera against selected refer­ence Mycoplasma spp. Whole-cell protein pat­terns generated by sodium dodecyl sulphate poly­acrylamide gel electrophoresis (SDS-PAGE) were used to identify and classify field isolates of a chosen Mycoplasma spp (19) and Acholeplasma spp (6). A high degree of similarity between most of the strains was established with strain-to-strain differences.

Main Subjects