IMMUNOLOGICAL STUDIES ON PORIN PROTEINS OF PASTEURELLA HEAMOLYTICA IN GOAT

Document Type : Original Article

Authors

1 Bacteriology Department. Biotechnology Department

2 Animal Health Research Institute, Doki, Giza, Egypt

Abstract

This study was carried out on 120 goats, 45 of them suffering from pneumonia with its clinical symptoms, 55 of them were died and 20 were ap­parently healthy. The morbidity and mortality rates reached 22.5% and 27.5%, respectively. A total of 25 nasal swabs were collected from pneumonic goat for isolation and identification of the causative agent. P. haemolytica was isolated pure in 44%. Other bacteria isolated coupled with Staphylococcus aureus in 24% and 32% Esheri­chia coil. The protein profiles of the purified bac­teria was carried out using SDS poly acrylamide gel electrophoresis by comparing with molecular weight marker changed from 10 - 250 KDa. This study was conducted to characterize and identify the purity of major porin proteins in outer mem­brane with molecular weight of 38 - 42 kDa . Of pasteurella heamolytica usinb SDS-PAGE. In the active immunization assay, porin proteins 501.1g conferred 100% protection to challenged mice. In addition crud extract give 70% protection when challenged mice. Immunization with porin pro­teins of P.heaniolytica developed a significant in­crease in scrum antibody titre, as shown by en­zyme linked immunasorbent assay., Stability assay , and opsonophagocytic - killing assay.
 

Main Subjects

Veterinary Medical Journal (Giza)
Volume 53, Issue 2
April 2005
Pages 209-219

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