DETECTION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS ISOLATED FROM MASTITIC BUFFALOES MILK USING PULSED- FIELD GEL ELECTROPHORESIS (PFGE)

EL-MAHROUK, A; ZAKI, E

doi:10.21608/vmjg.2005.384672

DETECTION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS ISOLATED FROM MASTITIC BUFFALOES MILK USING PULSED- FIELD GEL ELECTROPHORESIS (PFGE)

Document Type : Original Article

Authors

A EL-MAHROUK ¹
E ZAKI ²

¹ Microbial Toxins Research Unit

² Buffalo Diseases and Research Department Animal Health Research Institute, Dokki, Giza

10.21608/vmjg.2005.384672

Abstract

A total of 196 milk samples were examined bacteriologically for detection of methicillin resistant Staphylococcus aureus during the period from January to October 2004 and results obtained showed high incidence of isolation of S. aureus (38.46%) from a total 13 strains of staphylococci (29.54%). All obtained isolates were identified using a commercial kit system (API STAPH system consists of: Carrying out 19 tests using the API STAPH strip). Antibiotic sensitivity test were done using five antibiotics, which are frequently used in mastitis therapy were chosen: penicillin-G, ampicillin, kanamycin, and cephalexine. The fifth compound was methicillin and results obtained showed no such strains could be found. S. aureus strains were susceptible to the antibiotics tested corn- prise 60 % (penicillin G), 80 % (kanamycin, cephalexine) and ampicillin (20 %). Pathogenicity of the isolates were discussed including both haemolysin titre and pathogenicity in mice. The results obtained revealed that majority of the S. aureus (80%) isolates were haemolytic with the maximum haemolytic activity (1/ 1024) and concerning pathogenicity in mice mortality rate was 100%. Genetic characterization of staphylococci by Pulsed Field Gel Electrophoresis (PFGE) analysis revealed no S. aureus resistance methicillin was recorded.

Main Subjects