DETECTION OF CORONAVIRUS ANTIGEN IN FAECAL SAMPLES OBTATNES FROM NEWBORN BUFFALOE CALVES .

Document Type : Original Article

Authors

1 Faculty of Veterinary Medicine, Cairo University Giza, Egypt.

2 Animal Heath Research Institute, Dokki. Giza, Egypt.

Abstract

Diarrhea remains one of the most important causes of 
calf mortality. An attempt has been made to gather
current relevant clinical information relating to 
the aetiology of calf enteritis especially that of |
viral origin. The association between coronavirus
infection and diarrhea in calves was studied by
Mebus and Coworkers (1973 a and 1973 b).
Methods of diagnosing coronaviral infections of cal-
ves were studied clinically and pathologically (Stair
et al., 1972; and Marin et al., 1974). Virological 
examination for coronavirus in calves Was studied by
Patel et al. (1982), where the virus was detected by 
indirect immunofluorescence and electron microscopy.
in this work, three different methods were used for 
detection of coronavirus antigen(s) in faecal samples
of newborn Egyptian buffalo calves suffering from
diarrhea .these were the Enzyme Linked Immunosorbent 
Assay (ELISA), the Antigen Sopt Test (AST) and the
immunoperoxidase (IP) technique.

Main Subjects