TYPING OF STREPTOCOCCI BY MEANS OF SPECIFIC ANTIBODY ADSORBED TO PROTEIN-A CONTAINING STAPHYLOCOCCI

Document Type : Original Article

Author

Department of microbiology, Faculty of Veterinary Medicine, Cairo University.

Abstract

Staphylococcus protein-A (SPA) is a cell wall consti-
tuent of Staphylococcus aureus which has an extra -
ordinary affinity for binding to the FC region of
immunoglobulin G (IgG) from various mammalian species.
Cell bound SPA is found to be useful as an adsorbent
for detection of antigen-antibody complex (Goding,
1978, Kessler, 1981 and Lindmark et al., 1983).
The precipitation test is widely used for typing of -
streptococci by means of their carbohydrate substance
or antigen "C" in their cell walls (Crusickshank et
al., 1975). This test however, more laborious and
time consuming as a routine test. A slide agglutina-
tion method is here described, that renders strepto-
coccal typing quick and easy to be performed. when
specific anti-streptococcal antibodies, were added
to a stabilized suspension of staphylococci , they 
became bound to the protein A on the cell wall via  
the FC structure of IgG (Kronvall and Williams ,1969
and Kronvall and Frommel, 1970); therefor ,orienta-
ting the Fab-located antibody- combining sites outwards 
(Lind et al., 1972). When streptococci  of the corresp
onding group are added, coagglutinin occurs with
the antibody coated staphylococci. Thus , this work was
done to determine the more economic ,rapid and  easy 
method for typing of the examined streptococci.

Main Subjects

Veterinary Medical Journal (Giza)
Volume 38, Issue 1
January 1990
Pages 45-51

Files

Share

How to cite

Statistics