BACTERIAL AND MOLECULAR CHARACTERIZATION OF PASTEURELLA MULTOCIDA IN INFECTED TURKEYS

Document Type : Original Article

Authors

1 Dept. of Research of Poultry Disease, Animal Health Research Institute

2 Dept. of Research of Biotechnology, Animal Health Research Institute

Abstract

The primary objective of the study reported here was to evaluate the effect of natural affection of P.multocida in Turkeys The bacteriological examination of five turkey herds in Giza and Beni Swef governorate was done to isolate and identify Pasteurella spp with special attention to P. multocida. Outer membrane protein (OMP) profiles of obtained field isolates were done to specify the strain with reference to protein marker (17 5-16.5 kda) The wasting effect of Pasteurellosis on turkey was also determined by comparting the total protein gm % in the turkey organs in each of healthy and pasteurellosed birds. Bacteriological examination showed that 154 out of 400 turkey organs were Pasteurella postive. 62 of them were P. multocida. Otherwise, 48 5% of blood serum samples were positive for Pasteurella and 18 of these were identified biochemically as P.multocida, Antibiogram studies of the isolated P.multocida indicated that colistin sulphate, trimethoprim, sulphamethoxacin, tetracycline, amoxycillin and enrofloxacin were the most effective drugs in vitro, The electrophoretic analysis of the outer membrane protein (OMP) of the P.multocida isolates by using SDS-PAGE  revealed that all P.multocida strains were nearly at 52 kd & 49 kd, where P. multocida of group I, II, III were 5. .

Main Subjects

Veterinary Medical Journal (Giza)
Volume 54, Issue 4
October 2006
Pages 899-912

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