ONE-STEP RT-PCR TRAGETING THE FUSION PROTEIN GENE FOR DETECTION OF BOVINE RESPIRATORY SYNCYTIAL VIRUS

Document Type : Original Article

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1 Department of Virology, Faculty of Veterinary Medicine, Sadat City, Menoufia University

2 Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, Konxville, Tennessee, USA.

Abstract

RT-PCR F targeting a fragment of the fusion protein (F) gene of bovine respiratory syncytial virus (BRSV) has been recommended to detect BRSV. This assay is used to be performed in two steps after RNA extraction: cDNA synthesis then PCR. In this study, cDNA synthesis and PCR have been performed in one step single tube. This modification may save time and effort with the same sensitivity and specificity.

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