DETECCTION OF ENTEROTOXOGENIC CLOSTRIDIUM PERFRINGENS TYPE A IN CAMEL MEAT

Document Type : Original Article

Authors

1 Department of Bacteriology, Animal Health Research Institute, Dokki, Giza

2 Department of Bacterial toxin, Animal Health Research Institute, Dokki, Giza

Abstract

Clostridium perfringens enterotoxin (CPE) is an important sporulation associated virulence factor in several illnesses of humans and domestic animals.in the present investigation 169 camel meat samples were collected from small butchers’ shop where C. perfringens type A was identified with an incidence of 33.7% only 5.9% had detectable enterotoxin. The meat samples tested had MNP per Gram values ranging from 0 to 35, determination of spore heat resistance for cpe positive C. perfringens type A isolates varied between logs 1.3 to log 5.07 at different time intervals. The current study has used PCR assays to detect cpe production in gene as a method for determining the enter toxigenicity of C. perfringens isolates. Results showed that PCR is suitable for detection of C. perfringens enterotoxin from raw meat.

Main Subjects