RAPID LABORATORY DIAGNOSIS OF CAMPYLOBACTER INFECTION USING IMMUNOFLUORESCENT ANTIBODY TECHNIQUE (IFAT)

Authors

1 Dept. Microbiology, Fac. Vet. Med. Giza Univ.

2 Dept. Microbiology, Fac. Vet. Med. Moshtohor. Banha Univ.

3 Dept. Vet. Medicine, Fac. Vet. Med. Cairo Univ.

Abstract

Campylobacter species are frequent etiological cause of bacterial diarrhoea all over the world (Blaser et al. 1979 and Walder, 1982). Since 1977, campylobacter jejuni has been increasingly recognized as a cause of sporadic and epidemic diarrhoea, not only in animals but also among humans (Jones et al., 1981). Also different campylobacter species have been incriminated as a cause of mastitis, infertility and abortion among various animal species (Safford, 1969). The cultural recovery of campylobacter species from clinical specimens has been a constant problem, firstly, because of the relatively low numbers of organisms in the clinical specimens and the usually present contaminants and secondary because of the possibility of cold injury on campylobacter cells, especially when cultured on antibiotic media (Humphrey and Gruickshank, 1985). For these reasons, it was the aim of the present investigation to compare and to evaluate the efficacies of immunofluorescent antibody technique (IFAT) and the conventional culturing procedures in definitive diagnosis of campylobacteriosis.

Main Subjects