THE VALIDITY OF ENZYME LINKED IMMUNOSORBANT ASSAY IN DETECTION OF SALMONELLA IN DRESSED POULTRY

Authors

1 Faculty of Vet. Med. Cairo University

2 Animal Health Research Institute-Dokki, Cairo

Abstract

Salmonellae which are endemic in many poultry flocks, may causing minimal poultry health problems. However during the slaughter of such poultry by assembly-line procedures encourages cross contamination particularly in the slaughter operation with continuous water immersion chilling often takes place This fact explains why the poultry industry has received most of the blame for contamination of the food chain with salmonellae. Traditional methods for the isolation of salmonellae from poultry which involve non-selective pre-enrichment followed by secondary enrichment in selective broth media and subculture onto selective differential agars, have been used by many authors, (Wassef et al., 1980; El-Mossalami et al., 1986 and Nouman et al., 1986). Such methods are both labour, intensive and time consuming while a sample can provisionally be declared salmonella positive within 3 days, a final answer on a negative sample can take up to 5 days. Therefore a wide interest in a more rapid methods for the detection of salmonella and a number of procedures of varying complexity have been put forward. These include; the use of immunofluorescent technique (Safwat et al., 1981) Enrichment serology, radiometry, enzyme immunoassays and the used of gene probes (Emswiler et al., 1984; Rigby 1984; and Todd et al., 1986). The trend in this study is to verify the suitability of the enzyme immunoassay as rapid test for the detection of salmonellae in dressed broilers.

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