ISOLATION AND CHARACTERIZATION OF VARIANT STRAINS OF MYCOPLASMA GALLISEPTICUM (MG) FROM BROILER BREEDER CHICKEN AND RATS

H, HEDIA; E, OUDA; A, HUSSEIN; H, SULTAN; S., ATTA; EL JAKEE, KJ

doi:10.21608/vmjg.2007.378548

ISOLATION AND CHARACTERIZATION OF VARIANT STRAINS OF MYCOPLASMA GALLISEPTICUM (MG) FROM BROILER BREEDER CHICKEN AND RATS

Document Type : Original Article

Authors

¹ The National Research Center, Dept. of Microbiology and Immunity Dokki, Giza

² Animal Health Research Institute, Dept. of Mycoplasma Dokki, Giza

³ Faculty of Vet. Med., Dept. of Virology and Microbiology Cairo Univ.

⁴ Faculty of Vet. Med. Dept. of Poultry Diseases Minofya Univ.

10.21608/vmjg.2007.378548

Abstract

The serological profile of Mycoplasma gallisepticum (MG) in a broiler breeder flock showed negative result until 30 weeks of age, where it became MG positive ( 100%), when tested at both of 40 and 55 weeks of age. Mycoplasma could be detected from tracheal swabs in this broiler breeder flock at age 49 weeks by culture and polymerase chain reaction (PCR) .Isolation and characterization of the recovered isolates using culture, biochemical, serological and PCR testes proved to be MG. Dot ELISA was applied in the current study and it was potential enough in rapid confirmation of Mycoplasma isolates. Histopathological examination of the trachea and lungs of infected broiler breeder flock, revealed the destructive effect of MG. In a trial to investigate shedding of MG in four hatched chicks from the infected broiler breeder flock,
the result revealed that the examined hatches were negative by PCR, culture and serology. The observed number of rats in the MG infected broiler breeder farm directs the attention for the role of rats in transmitting MG. Trials for isolation and characterization of Mycoplasma from such rats were conducted and the isolate was proven to be MG strain. A pathogenicity study was carried out on the two MG isolates (broiler breeder and rat strains) in SPF chicks. The results showed that, the two isolates were different in their effect reflecting the variable behavior of MG in different species or highly raise the presence of a mutant or variant strain of MG, with variable degree of pathogenicity in chickens. The study reports, the isolation and characterization of MG from rats and chicken with different pathogenicity. Also, the presence of MG in rat

Main Subjects