PRODUCTION OF MONOCLONAL ANTIBODIES AGAINST BOVINE PARVOVIRUS

Document Type : Original Article

Authors

1 Animal Health Research Institute, Dokki, Giza, Egypt.

2 Dept. of Virology, Faculty of Vet. Med., Cairo University, 12211 Giza, Egypt.

Abstract

Monoclonal antibodies (MAbs) against bovine Parvovirus (BPV) were successfully produced after fusing the spleen cells of an immunized mouse with myeloma cells. A total of six hybridomas were found to produce MAbs against BPV with different” reactivilles Using heamagglutination test. these clones are: 3B10, 3c5,3D6, 2H6 and 1E6. the first three clones produced MAbs of IgG class, but the last three clones produced those of the IgM class and all of them contained K light chain.

Main Subjects