EVALUATION OF SOME DIAGNOSTIC TESTS FOR BRUCELLOSIS IN CATTLE

Authors

1 Dept. OF Animal Medicine Faculty. of Vet. Med. Zagazig University, Egypt

2 Dept. OF serum and Antigens Veterinary Serum and Vaccine Research Institute, Abbassia ,Cairo , Egypt

Abstract

The present study was carried out on 442 cattle of which 238 lactating 176 non lactating 9 aborted cows, 6 calves and 13 bulls of Eriezian breed in a governmental farm at Sharkia Governorate and had a previous history of brucellosis; abortion and retained palcenta. Blood sera from the examined cattle were subjected to buffered acidified plate antigen (BAPA), Rose Bengal plate (RBPT), Enzyme linked immunosorbent assay (ELISA), Complement fixation test  (CFT), Tube agglutination test (TAT). In general, the positive reactors were 28.51 %, 28.05 %, 24.89%, 22.85% and 21.72% while the postitive percentage in lactating cows 29.83 %, 29.41 %, 24.79 %, 22.27% and 21.85 % while in non lactating cows the Percentage of positive reactors were 25.0 %, 22.73%, 21.02 % and 18.75 % with BAPA, RBPT,ELISA, CFT and TAT respectively. Application of milk ring test (MRT) on milk from 238 individual lactating cows revealed that 39 (16.39 %) were positivbe reactors. Relative agreement (concordance) between different serological tests were estimated and discussed. Trials for isolation of brucella from milk samples using guinea pig inoculation revealed negative results.

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