ELECTRORETIC DIFFERENTIATION OF SOLUBLE ANTIGENS FROM ECHINOCOCCUS GRANULOSUS ISOLATES USING SDS-PAGE TECHNIQUE

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Parasitology and Animal Diseases Dept., National Research Centre, Dokki. Giza, Egypt.

Abstract

a comparison Was made by sodium dodecyle polyacrylamide gel electrophoresis SDS-PAGE between somatic hydatid fluid, Protoscoleces and Echinococcus granulous adults antigens recovered from camel and equine (donkey) origins. Comparing hydatid fluids indicated that differences were found in the arrangement and molecular weights of developed protein bands. Donkey hydatid fluid showed 3 protein bands having a molecular weight ranging between 44.6-143 K Da. while that of camel origin revealed 7 bands. These bands had variable molecular weights either low (28.8 - 46.7 K Da.) or high (72.4 - 100.0 K Da.). The protein patterns of protoscoleces were most complex in both origins, Protein experession in camel Protoscoleces had 12 bands. These bands had Variable molecular weights, 8 bands having low molecular weights (19.9 - 64.6 K Da.), the other 4 bands had higher molecular weights (89.1 - 136.3 K Da.). The identified bands among donkey protoscoleces were 8 bands. Only one band had a low molecular weight (53.7 K Da.). while the others were of high molecular weights ‘67.6 - 157.9 K Da.). Protein profile of E. granulosus somatic antigens revealed that both isolates had equal number of protein bands. All the stained protein bands were relatively similar except two bands having low molecular weight 10.5 & -6.3 K Da. However, one band characterized camel sample had a high molecular weight (105.3 K Da.).
 

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