TESTING OF THE EGYPTIANCATTLE AND WATER BUFFALOES (BUBALUS BUBALIS) FOR PARAFILARIA BAVICOLA USING ELISA

Authors

1 Department of Parasitology, Faculty of Veterinary medicine, Cairo university

2 Departement of Parasitology, National Veterinary Institute, Uppsala, Sweden

Abstract

A total of 411 sera (323 from cattle and 88 from water buffaloes) 2 to 15 years old were collected from different localities from Egypt (Cairo, Delta and Upper Egypt). The sera were tested by ELISA for Parafilaria bovicola. Monoclonal mouse anti-Parafilaria bovicola directed to 41 kDa protein (National Veterinary institute, Uppsala, Sweden) has been used. All the tested Sera recorded negative results. The inhibition rate was recorded to be 25-30% in cattle and buffaloes from Cairo. While, in Delta and Upper Egypt it was recorded to be 14.9 to 21.3%. the older ages (10-15 years) recorded higher inhibition rate (28.3-30.8%). The standard deviation was evaluated at 95%.
 

Main Subjects