DETECTION OF RINDERPEST VIRUS BY POLYMERASE CHAIN REACTION (PCR) AND SOUTHERN BLOT HYBRIDIZATION

SABER, M.S.; ABBAS, A.M.

doi:10.21608/vmjg.1998.377240

DETECTION OF RINDERPEST VIRUS BY POLYMERASE CHAIN REACTION (PCR) AND SOUTHERN BLOT HYBRIDIZATION

Authors

M.S. SABER ¹
A.M. ABBAS ²

¹ Faculty of Veterinary Medicine, cairo University, Giza, Egypt

² Genetic Engineering Unit, Veterinary Serum and Vaccine Research Institute, Abasssia, Cairo, Egypt

10.21608/vmjg.1998.377240

Abstract

Rinderpest Is an economically highly important disease affecting cattle and buffalo. It's endemic throughout large parts of Africa, Asia, and the Middle East (Egypt). The reverse transcription reaction (RT-PCR), using phosphoprotein (P) gene specific primers set, was used to detect rinderpest virus extracted from infected VERO cells. The amplified 429 bp PCR product was subjected to digestion with restriction endonucleases enzymes (Alu 1 & Hae III) which cut at specific sequence sites derived from rinderpest phosphoprotein (P) gene. The PCR product served as a probe for detection of cDNA derived from infected VERO cells with rinderpest, so PCR assay when combined with southern blot hybridization hopefully will pave the way for rapid detection, monitoring, and surveillance of rinderpest infection.

Main Subjects