Nine cytopathic bovine rota virus stratins were isolted in MA104 cells from fecal specimens of calves. Plaque assay was used for selection, titration and purification of small and large plaques of selected rota virus strains. Electron microscopy was used to detect the presence of particles with rota virus morphology in MA104 cells. Virus neutralization test was used for further identification of strains of high immunogenic response.
Pooling of these isolates and their use in making a formalized-inactivated bovine rota vaccine was tried.
WASSEL, M. (1996). ISOLATION AND PREPARATION OF BOVINE ROTA VIRUS (BRV) VACCINE IN MINNESOTA, USA. Veterinary Medical Journal (Giza), 44(3), 583-591. doi: 10.21608/vmjg.1996.376625
MLA
M. WASSEL. "ISOLATION AND PREPARATION OF BOVINE ROTA VIRUS (BRV) VACCINE IN MINNESOTA, USA", Veterinary Medical Journal (Giza), 44, 3, 1996, 583-591. doi: 10.21608/vmjg.1996.376625
HARVARD
WASSEL, M. (1996). 'ISOLATION AND PREPARATION OF BOVINE ROTA VIRUS (BRV) VACCINE IN MINNESOTA, USA', Veterinary Medical Journal (Giza), 44(3), pp. 583-591. doi: 10.21608/vmjg.1996.376625
VANCOUVER
WASSEL, M. ISOLATION AND PREPARATION OF BOVINE ROTA VIRUS (BRV) VACCINE IN MINNESOTA, USA. Veterinary Medical Journal (Giza), 1996; 44(3): 583-591. doi: 10.21608/vmjg.1996.376625
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