IMMUNODETECTION OF AMPLICAECUM LARVAL HaxsMOGLOBIN USING ELISA TECHNIQUE

Document Type : Original Article

Authors

1 Fish Diseases Department, Animal Health Research Institute, Dokki, Giza, Egypt.

2 Faculty of veterinary Medicine, Cairo Univ.

Abstract

Amplicaecum larvae are a significant parasite infesting Tilapia and some predatory fish species at Nasser Lake since 1988. these laval stages were homogenized and prepared as a crude antigen (structural antigen). The protein concentration was estimated by monitoring the UV absorption Spectrum. The positive immune serum anti Amplicaecum larval haemoglobin was prepared and sensitiized in vivo. The highest sensitivity of the haemoglobin was estimated by ELISA technique and amounted to a titer of 1/20 with optical density x 0.554.

Main Subjects