The recombinant protein (r1448) in crude lysate of insect cells infected with a previously devcl-oped recombinant baculovirus, expressing two structural protcins, the nucleocapsid protein (p14) and the envelope glycoprotein (gp48) of the NADL strain of bovine viral diarrhea virus (BVDV), was evaluated as a coating antigen in a Standard enzyme-linked immunosorbent assay (ELISA) for detection of antibody against homol- ogous and heterologous strains of BVDV. The ELISA was compared to standard virus neutrali-zation test (VNT) for detection of antibody against the local Iman strain of BVDV in sera of calves immunized with the locally produced Pneumo-3 inactivated vaccine, experimentally in- fected calves and contact controls. Antibody titers With ELISA were closcly corrclatcd ‘ibody titers estimated by VNT (averager= 0.917). Nevertheless, thc recombinant protcin (r1448) was an effective and inexpensive ELISA antigen for detection of antibody against homolo- gous and heterologous strains of both gcnotypcs of BVDV (BVDVI and BVDV2) In reference hyperimmune cattle scra. In conclusion, the recombinant antigen (r1448) is a reliable candi-date for diagnostic tests of BVDV infections in Egypt.
EL-KHOLY, A., GHALY, H., SAID, S., & EL-SABBAGH, M. (2000). EVALUAT ION OF A RECOMBINANT ELISA ANTIGEN FOR SERODIAGNOSIS OF BOVINE VIRAL DIARRHEA IN IMMUNE AND INFECTED CATTLE SERA IN EGYPT. Veterinary Medical Journal (Giza), 48(2), 227-238. doi: 10.21608/vmjg.2000.376270
MLA
A EL-KHOLY; H GHALY; SAMIRA SAID; M EL-SABBAGH. "EVALUAT ION OF A RECOMBINANT ELISA ANTIGEN FOR SERODIAGNOSIS OF BOVINE VIRAL DIARRHEA IN IMMUNE AND INFECTED CATTLE SERA IN EGYPT", Veterinary Medical Journal (Giza), 48, 2, 2000, 227-238. doi: 10.21608/vmjg.2000.376270
HARVARD
EL-KHOLY, A., GHALY, H., SAID, S., EL-SABBAGH, M. (2000). 'EVALUAT ION OF A RECOMBINANT ELISA ANTIGEN FOR SERODIAGNOSIS OF BOVINE VIRAL DIARRHEA IN IMMUNE AND INFECTED CATTLE SERA IN EGYPT', Veterinary Medical Journal (Giza), 48(2), pp. 227-238. doi: 10.21608/vmjg.2000.376270
VANCOUVER
EL-KHOLY, A., GHALY, H., SAID, S., EL-SABBAGH, M. EVALUAT ION OF A RECOMBINANT ELISA ANTIGEN FOR SERODIAGNOSIS OF BOVINE VIRAL DIARRHEA IN IMMUNE AND INFECTED CATTLE SERA IN EGYPT. Veterinary Medical Journal (Giza), 2000; 48(2): 227-238. doi: 10.21608/vmjg.2000.376270
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