COMPARISON BETWEEN SOLID PHASE ELISA, DOT ELISA AND AGAR GEL PRECIPITATION TEST FOR DETECTION OF BURSAL DISEASE VIRAL ANTIGEN IN BURSAL HOMOGENATES

Document Type : Original Article

Authors

1 Ass. Prof. of virology, Fac. Vet. Med.; Beni-Suef, Cairo Univ.

2 lecaturer of Poultry Dis., Fac. Vet.. Med; Cairo University.

Abstract

Forty-four bursal samples were collected from naturally infected and normal broiler chicks. The samples were homogenized and the homogenates were tested by agar gel precipitation test (AGPT), solid-phase ELISA (by indirect double antibodies sandwitch and single antibody ELISA systems) and Dot ELISA for detection of infectious bursal disease virus (IBDv) antigen. The reacted positive samples showed 86.3,88.6 and 88.6 percentage in AGPT, solid-phase ELISA and Dot ELISA, respectively. The findings suggest that the Dot ELISA is faster, more economic and easily applicable procedure than the other two techniques. The indirect double antibody sandwitch technique-is more sensitive than the indirect single antibody EIISA system.

Main Subjects