STOMET AND OESTRAD oF NORGE IOL VALERA USE PNORGESTOMET IMPLANT AND / OR PMSG ATE INJECTION Wn OESTRUS IN ANOESTROUS BUFFALOES DUCTION

The study was designed to evaluate 5 hormonal treatments for induction of oestrus in anoestrous buffalocs . Treatment  of subcutaneous 3 – mg noreestomet implant and an intramuscular injec- tion of 3 mg of norgestomet and 5 mg estradiol Valerate, with implant removed 9 days later (N=15; norgestomet - estradiol) ; Treatment 2 of Norgcstomet - estradiol, with an intramuscular in-Jection of S00 IU of PMSG at the time of implant“moval (n=13) ; Treatment 3 of intramuscular "ection of 500 IU PMSG (n=10) ; Treatment 4of intramuscular injection of 1500 IU PMSGmee ene 5 of two intramuscular injec-imals ceca (500 TU) 3 days apart (n=5). Tened no treatment and used as a con-Yol proseus "P. The Proportions of animals exhibitedrus during 35 qdn] 5 Were 6.7Mtly. No animays after treatment 1 ,2,3,4,» 16.9 , 60 , 30 and 100 % respectine fy al in control group came in ocstrusriN'"S Perio .a, Conception rate after natural "ting al ingIn uc¢e ° < d oestrus in the five treatments 135 was 100 % . The highcst ocstrous rate (100%) and the shortest interval between treatment and displayed oestrus ( within S days ) was obtained aficr treatment 5 . Progestcrone concentration was measured in all sainplcs collected before treat-ment and sera collected from 5 animals did not exhibit oestrus after treatment | and from 5 ani-mals exhibited ocstrus 3 days after treatment 2 for monitoring ovarian status before and _ after treatment. Progesterone concentrations was < 0.22 ng /ml in two samples collected from all animals before treatment. Mcan progestcrone concentrations were maintained at low basal Ievels (0.18 + 0.09 - 0.21+ 0.07 ng/ ml) after treatment |however, it raised to 2.56 + 0.61 ng/ml at day 7 post treatment and maintained at high levies (2.80 + 1.68 - 4.414 0.94 ng/ml ) during 28 days after treatment 2.