IMMUNOGENIC PROPERTIES OF OUTER MEMBRANE PROTEIN OF CAMPYLOBACTER JEJUNI IN CHICKS

Document Type : Original Article

Authors

1 Serology Unit, Animal Health Research Institute, Dokki, Giza

2 Dept. of Parasitology and Animal Diseases, National Research Center, Dokki, Giza

Abstract

The immune response of chickens to outer membrane proteins (OMP) of Campylobacter jejuni (C. jejuni) was studied as a step in the search for Vaccine candidates. OMP of C. jejuni was administered I/M to native breed chicks at one Month of age for 4 times weekly intervals. Serum samples were collected for 4 successive weeks And immune response was measured by enzyme Linked immunosorbent assay (ELISA). Experimental chicks were challenged with 5 x 108 C. jejuni orally after one week of last vaccination. At The end of the experiment, the reisolation of C. jejuni was carried out and specimens for histopathOlogical examination were taken. The immunized Experimental birds showed increase in titres of Specific antibodies in sera than control group. The immunized and challenged experimental birds (group 1) had lower reisolation of C. jejuni (16.66%) than unimmunized challenged control Group (group 2) (85%), while there was no reisolation in control negative group (group 3). Histopathological examination revealed round cell aggregation and mild to moderate inflammatory changes in liver and intestine of group 1 while more severe degrees of inflammatory changes in Group 2. The results indicate that immunization can reduce the level of infection with C. jejuni But not completely protection.

Main Subjects

Veterinary Medical Journal (Giza)
Volume 51, Issue 1
January 2003
Pages 95-104

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