EFFECT OF THIOUREA ON MOTILITY AND VIABILITY OF CHILLED-STORED AND FROZEN-THAWED GOAT SPERMATOZOA

Document Type : Original Article

Authors

Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12515, Egypt.

Abstract

The aim of the present study was to elucidate the effect of thiourea on motility and viability of chilled-stored and frozen-thawed goat spermatozoa. Five male Baladi goats were used in three experiments during this work. Three different Concentrations (10,50 and 100 mg %) of thiourea were added to semen extenders before processing. Tris-based egg yolk and Tris-egg yolk free extenders were used for chilled-storage of spermatozoa in the first and second experiments, respectively. In the third experiment, motility and viability of frozen-thawed goat semen were evaluated after inclusion of 50 mg% thiourea in the extender. In vitro augmentation of semen extender with 50 mg % thiourea exerted a pronounced improvement in incubated and post-thawed sperm motility percentages and viability indices of goat spermatozoa either in presence or absence of egg yolk. It is concluded that Tris-egg yolk free extenders could be used in presence of 50 mg % thiourea for both incubation and preservation goat semen.

Main Subjects