On the end of December 2001, mild local exanthemas and generalized pox-like lesions were observed in dromedary camels herd at Badrashin Locality Giza Governorate. Skin lesions (scabs&crusty materials) were scraped from the Nose, upper and lower lips, upper and lower parts of the neck and the external sides of the hind Limbs of affected camels (2-3 years old) then prepared aseptically for virus isolation on VERO cells and chorio-allantoic membrans (CAMS) of Embryonated chicken eggs(ECE). 54 serum samples were collected randomly from apparently healthy camels found in the neighbourIng villages, and tested by Serum Neutralization Test (SNT) and Agar Gel Precipitation Test (AGPT) for detection of specific neutralizing and Precipitating antibodies of camel pox virus. The results revealed the isolation of camel pox virus (CPV) from skin lesions on VERO cells and CAMS of ECE. The virus was identified by virus Neutralization test VNT and AGPT using reference camel pox hyperimmune serum. 8 out of 54 Sera sample were positive by SNT with percent of 14.8 where as only 3 samples were positive in AGPT with percent of 5.6. The isolated camel pox virus was designated as Badrashin isolate. The grouth behavior on VERO Cells and ECE of the virus and its haemagglutinatIng activity were studied and compared with the Previous Marsamatroh isolate. The results indicated that both isolates were considerably similar.
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