THE EFFECT OF PREPARED IMMUNOGLOBULINS RICH FRACTIONS AGAINST EXPERIMENTAL ESCHERI- CHIA COLI INFECTION IN CHICKENS

Document Type : Original Article

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National Research Center , Dokki , Egypt Fac. of Vet. Med. ,Cairo University

Abstract

The optimal concentration of ammonium sulphate required for precipitation of maximal amounts of chicken immunoglobulin (Ig) was determined. The third precipitate and first supernatant ‘fluid obtained from treatment of pooled chicken serum with 25, 30, 35, 40, 45 and 50% final concentrations of ammonium sulphate were electrophoretically analyzed in poly-acrylamide gels as well as immuno electrophoretically examined against anti-chicken serum prepared in rabbits. 30% ammonium sulphate concentration was optimal for precipitation of total immunoglobulins from chicken serum, 25% for precipitation of IgM and 45% for precipitation of IgY.
When serum IgM or IgY-rich fraction preparation was given 24 hours prior to E-coli (serogroup 078: k 80 infection, protected the experimental birds (about 3: times) against mortality and persistence of the inoculated pathogen than infected no pretreated groups, regardless of the route of administration or class or dose o: Ig used. The degree of protection proves to be dose, route and Ig-class dependent.

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