MODULATORY EFFECTS OF BUFFALO BASIC AND ACIDIC UTERINE LUMINAL PROTEINS ON PHAGOCYTIC AND BACTERICIDAL ACTIVITIES OF POLYMORPHONUCLEAR- AND MONONUCLEAR LEUKOCYTES IN VITRO

RAMADAN, A; ZAKI, A; HASSAN, H; HASHAD, M

doi:10.21608/vmjg.2001.372282

MODULATORY EFFECTS OF BUFFALO BASIC AND ACIDIC UTERINE LUMINAL PROTEINS ON PHAGOCYTIC AND BACTERICIDAL ACTIVITIES OF POLYMORPHONUCLEAR- AND MONONUCLEAR LEUKOCYTES IN VITRO

Document Type : Original Article

Authors

¹ Immunobiology and Immunopharmacology Unit: Biology of Reproduction Department , Animal Reproduction Research Institute, P.O. Box 12556, Giza, Egypt

² Microbiology Dept. Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

10.21608/vmjg.2001.372282

Abstract

Potential immunological effects of basic and acid- ic uterine luminal proteins (ULP) collected from estrus- and luteal-phase buffaloes were studied in vitro. The phagocytic and microbcidal activities of PMN and macrophages, nitric oxide production by macrophages, and superoxide anion production by PMN were elucidated. Estrus-phase basic and acidic ULP collected from buffaloes enhanced phagocytic activity of PMN at all concentrations tested, whereas basic and acidic ULP of luteal- phase buffaloes suppressed phagocytic activity in a dose-dependent manner. The bactericidal activ- ity of buffalo PMN increased in response to basic ULP of estrus-phase buffaloes in comparison with basic luteal-phase proteins. Meanwhile, acidic ULP proteins of estrus-phase buffaloes showed significant increase in bactericidal activity only at 40, 80, and 150 μg/ml protein concentrations in contrast to acidic ULP of luteal-phase buffaloes. Nitric oxide production by macrophages in- creased in response to basic ULP of estrus-phase buffaloes, whereas this activity decreased when macrophages were treated with different concentrations of basic ULP of luteal-phase buffaloes. Only at high concentrations the acidic protein of estrus-phase buffaloes increased nitric oxide production by macrophages as compared with acidic proteins from luteal-phase buffaloes. Superoxide anion productions by neutrophils increased in response to basic and acidic ULP of estrus-phase buffaloes and decreased in response to acidic pro- teins from luteal-phase buffaloes. These data demonstrate that buffalo uterus contains immunologically active secretory proteins that can influence the natural defense barriers of the buffalo genital tract.

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