PREPARATION AND LABORATORY EVALUATION OF LIVE ATTENUATED INFECTIOUS BURSAL DISEASE (IBD) VIRUS VACCINE FROM RECENT EGYPTIAN ISOLATE PROPAGATED ON CHICKEN EMBRYO FIBROBLAST (CEF) CELL CULTURE.

Document Type : Original Article

Authors

1 Faculty of Veterinary Medicine, Cairo University, Beni-Suef branch.

2 Veterinary Serum and Vaccine Research Institute, Abbassia. Cairo.

Abstract

Very virulent IBDV was propagated for 3 passages in SPF-ECE followed by 3 passages in young susceptible chicks then for 3 passages in SPF. ECE then for further 12 passages in SPF-ECE. The first passage of last propagation was further propagated for 3 passages on Vero cells and for 60 passages in CEF-cell culture. The passage 40 of propagated IBDV proved to be safe and highly immunogenic by virus neutralization test. It still elicited high neutralizing antibody titers (18 log2) for a stationary phase about 7 months (the end of the experiment) and high values of lymphocyte blastogenesis (0.560 versus control 0.03). Vaccinated chicks well protected against challenge with highly virulent IBDV after 3 weeks post-vaccination (PV). The prepared vaccine has superior potential immunogenic effect than commercial live mild, and intermediate plus (hot) vaccines. The vaccine is effective even when preserved for 8 months (the end of ex- - periment) at -20°C.

Main Subjects

Veterinary Medical Journal (Giza)
Volume 49, Issue 4
October 2001
Pages 563-573

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