PREPARATION AND EVALUATION OF AN INACTIVATED INFECTIOUS BURSAL DISEASE (IBD) VIRUS VACCINES FROM RECENT EGYPTIAN VIRUS ISOLATE ADJUVATED WITH NIGELLA SATIVA OIL.

Document Type : Original Article

Authors

1 Faculty of Veterinary Medicine, Cairo University, Beni-suef branch.

2 Serum and Vaccines Research Institute, Abbassia. Cairo.

Abstract

Inactivated IBD virus vaccines were prepared from a recent Egyptian isolate and adjuvated with Nigella sativa oil. The first passages of propagat ed viruses in SPF- embryonated chicken eggs ( ECE), Vero and chicken embryo fibroblast (CEF ) cell cultures were inactivated with binary ethy- lenemine (BEI), and supplemented with Negilla sativa oil as adjuvant The prepared vaccines proved to be highly immu- nogenic and elicited high titers of neutralizing an- libodies (17-20 log 2 )at weekly interval till 7 months post-vaccination (PV) and high values of lymphocyte blastogenesis (0.598 versus control 06). Besides they were able to protect vaccinat- ed chickens (100 % protection) when challenged Days PV. The superior potential effect of these vaccines, when compared with imported one, may due to the use of recent local IBDV isolate and Nigella sativa oil for its non specific immune stimulation effect. In addition, the keeping quality of prepared vaccines proved to be sterile, safe, stable and potent when preserved at 4°C for 6 months (the end of the experiment) as they pro- duced 100 % and 80 % protection after 3 and 6 months of preservation respectively. Each chick received 0.3 ml as one dose subcutaneously. From these studies we conclude that the use of Nigella sativa oil as adjuvant is considered a new trend in preparing inactivated vaccines.

Main Subjects

Veterinary Medical Journal (Giza)
Volume 49, Issue 4
October 2001
Pages 553-562

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