SUPEROVULATORY RESPONSE AND EMBRYO RECOVERY IN BUFFALO HEIFERS AND COWS TREATED WITH FSH (SUPER-OV) AND LH

Document Type : Original Article

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Dept. Theriogenology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt

Abstract

Superovulation and nonsurgical embryo collection were tried on 5 heifers and 16 buffalo cows. These animals were at mid luteal phase before treatment. Purified porcine FSH (Super-Ov) was administered intramuscular, morning and evening for 3 consecutive days. PGF20 (Lutalyse) 25mg was injected with the fifth Super-Ov injection. Chorionic gonadotropin (2000 USP unit) was injected in heifers and 10 of the cows during oestrus. The other six buffalo cows were considered as control without LH supplement. The onset and duration of oestrus were detected using buffalo bulls which frequently mated the females. Corpora lutea (CL) and unovulated follicles (UF) were counted rectally before non-surgical embryo col- lection. Oestrus started earlier and lasted longer in heifers than in cows and the differences were significant. The numbers of CL were significantly lower in heifers while the numbers of UF were Higher. The ovulation rates in heifers and cows were 36.6 ± 12.8% and 65.2 ± 8.4% respectively. In control buffalo cows, significant increase in the number of UF has been concomitant with significant reduction in the ovulation rate as compared to LH injected-cows (P<0.05). Uterine flushings from 10 and 6 animals revealed in the recovery of 12 and 6 embryos with a recovery rate of 27.9% and 23.8% in buffalo cows with and without LH respectively. The used protocol appeared satisfactory in buffalo cows rather than buffalo heifers and addition of LH improved ovarian response and embryo recovery.

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