ISOLATION AND IDENTIFICATION OF THREE-DAY SICKNESS VIRUS IN EGYPT

Document Type : Original Article

Authors

Department of Virology, Animal Health Research Institute, Dokki-Giza

Abstract

In summer 2000 several outbreaks among imported cattle with clinical manifestations similar to Bovine Ephemeral Fever (BEF) have been recorded in different Governorates in Egypt. 186 heparinized blood samples were collected for virus isolation and identification and 213 serum samples For serological examination. Suckling baby mice were intracerebrally (I/C) inoculated and Vero, BHK21 cells were also infected with buffy coat for viral isolation then indirect. Fluorescent antibody test (IFAT) was applied to Identify the BEF virus in impression smear of Baby mice brain and inoculated Vero and BHK21 Cells. So serological investigation was carried out to clear Up the immune status in infected cattle.

Main Subjects