Nine tissue cultures, 7 media for virus propagation and 5 lots of bovine sera as enrichment were brought to the Mycoplasma research department and routinely examined for contamination with Mycoplasma Four M. hominis were isolated from cell cultures, 3 M. salivarium were isolated from culture media while bovine serum samples revealed 3 bovine mycoplasmas isolates, 2 of them were M. arginini and one as A. Laidlawii. Antibiotic sensitivity test for Mycoplasma isolates was applied against 8 antimicrobial agents using metabolic inhibition test (MIT) and growth inhibition test (GIT) and spectinomycin proved to be the most effective and was recommended for treatment of contaminated tissue cultures. A trial was made to investigate the source of contamination. Out of 10 laboratory personnel 7 (.70%) Mycoplasma salivanum were isolated from throat samples while 6 (60%) were isolated when they were allowed to talk in front of plates held 2 inches from the mouth.
EL-SHABINY, L., KARIM, I., & S, E. (1992). MYCOPLASMA AS CONTAMINANT OF CELL QULTURE WITH SPECIAL REFERENCE TO SOURCE OF INFECTION. Veterinary Medical Journal (Giza), 40(2), 43-47. doi: 10.21608/vmjg.1992.372191
MLA
LAILA EL-SHABINY; IKRAM KARIM; EL-NAKASHL S. "MYCOPLASMA AS CONTAMINANT OF CELL QULTURE WITH SPECIAL REFERENCE TO SOURCE OF INFECTION", Veterinary Medical Journal (Giza), 40, 2, 1992, 43-47. doi: 10.21608/vmjg.1992.372191
HARVARD
EL-SHABINY, L., KARIM, I., S, E. (1992). 'MYCOPLASMA AS CONTAMINANT OF CELL QULTURE WITH SPECIAL REFERENCE TO SOURCE OF INFECTION', Veterinary Medical Journal (Giza), 40(2), pp. 43-47. doi: 10.21608/vmjg.1992.372191
VANCOUVER
EL-SHABINY, L., KARIM, I., S, E. MYCOPLASMA AS CONTAMINANT OF CELL QULTURE WITH SPECIAL REFERENCE TO SOURCE OF INFECTION. Veterinary Medical Journal (Giza), 1992; 40(2): 43-47. doi: 10.21608/vmjg.1992.372191
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