ISOLATION AND IDENTIFICATION OF CANINE ADENOVIRUS

Document Type : Original Article

Authors

Department of Virology, Animal Health Research Institute

Abstract

Eleven rectal swabs and 3 liver samples were collected from young dogs which were suffering From abdominal pain, tenderness, tympani and Bloody diarrhoea with or without vomition and Cough from private clinic and EL-Kanater area. Six cases of rectal swabs and 2 cases of liver samples were positive for adenovirus. Isolation on MDBK cell culture and identified by Complement Fixation test (CFT) and indirect immunoflourescent test (IFAT) using reference antiserum of bovine adenovirus type 1. Haemagglutination when Applied gave positive result against rat RBCs and Human RBCs type “O” only. Histopathological assay was done for detection of Basophilic inclusion in infected tissue culture Stained with H and E stain.

Main Subjects