USE OF MILK PROGESTERONE RADIOIMMUNO ASSAY AND IMMUNOASSAY IN TEH DIAGNOSIS OF PREGNANCY IN BUFFALOES

Document Type : Original Article

Authors

1 Department of Theriogenology ,Faculty of Veterinary Medicine, Cairo University.

2 Department of Biochemistry , Faculty of Veterinary Medicine, Cairo University.

3 Department of Physiology , Faculty of Veterinary Medicine, Cairo University

Abstract

In view of comparing between milk progesterone radio immunoassay and enzyme immunoassay for diagnosis of pregnancy in buffaloes, 140 whole samples were collected from 20 cows on 18th. 19 h, 20h, 21th 22nd, 23rd and 24th after mating. The milk samples were analyzed for (progesterone with RIA and EIA, Applicability, reliability and the values of progesterone measured by both methods were compared. This study indicated that day 20th, 21th of 22nd is the most reliable for performing this analysis for pregnancy diagnosis in in buffaloes.

Main Subjects