GENTAMICIN AND NETILMICIN RESIDUES IN TISSUES AND ORGANS OF TREATED RABBITS

AMER, M; ABD ALLA, WAFFIA; KOSHAIK, M; ABDEL ALEAM, A; GAMMAZ, H

doi:10.21608/vmjg.1994.372059

GENTAMICIN AND NETILMICIN RESIDUES IN TISSUES AND ORGANS OF TREATED RABBITS

Document Type : Original Article

Authors

¹ Dep. Pharmacology, Fac. Vet. Med., Suez Canal Univ.inderpest Vaccine Department. Serum and Vaccine Research Institute, Abbassia, Cairo.

² Dep. Food Hygiene, Animal Health Research Institute, Dokki, Cairo

³ Dep. Pharmacology, Fac. Vet. med., Zagazig Univ.

⁴ Dep. Pharmacology, Fac. Vet. Med., Suez Canal Univ.

10.21608/vmjg.1994.372059

Abstract

Thirty Balady rabbits of both sexes (Weighing 1.5-2 kg.B.Wt.) were used for studying gentamicin and netilmicin residues. The effects of heat treatment and freezing on the presence of these residues were also studied. The drugs were injected 1/M (6 mg/kg B. Wt.) daily for 7 successive days. Animals were slaughtred at different intervals, samples from shoulder, thigh and back muscles, heart, liver and kidneys were examined microbiologically for detecting the residues. Parts from samples of the animals containing the highest amount of residues (slaughtered at 12 hours after treatment) were boild for 45 minutes and tested for the presence of residues, while the rest of these samples (12 hours) were freezed and examined weekly for the presencc of the tested drugs. It was achieved that traces of gentamicin or netilmicin residues could be detected in the muscle samples up to 24 hours post last injection (0.17, 0.13 ppm respectively). The residues also disapeared by boiling the samples for 45 minutes and also by freezing for one week . The least amount of residues were detected in the heart (0.07 ppm). Liver samples of both drugs contained higher levels of residues that disappeared after 48 hours following drug injection (0.3 ppm). Kidney samples of both drugs showed the highest level of residues which disappeared after 4 days for gentamicin (0.75 ppm) and 5 days for netilmicin (0.9 ppm). Moreover, boiling of kidney or liver samples for 45 minutes did not destroy the residues completely. Furthermore, freezing for 3 months also failed to destroy the residues in the kidncys. It is concluded that gentamicin and netilmicin were highly concentrated in the kidneys followed by the liver while taces were present in the mu cles which disappeared after 24 hours of injection and by boiling.

Main Subjects