STUDIES ON MICRO ORGANISMS CAUSING BOVINE MASTITIS WITH PARTICULAR REFERENCE TO ANAEROBES

Document Type : Original Article

Authors

1 Animal Health Research Instirute , Dokki , Giza , Egypt.

2 Microbiology Dept. Fac. Vet.Med. Cairo Univ.(Beni-Suef Branch).

Abstract

The incidence of strict anaerobes presents alone or combined with other facultative anaerobes causing mastitis among cows was 34.9% C. perfringeus (4.1%), B. fragilis (3.7%), E. leatum
(3.3%), F. aecropharoum (3.3%) and anaerobes (3.3%) secured the more frequently obligatory anaerobic organisms Causing mastitis in cows either in pure form or combined with facultative anaerobes.
 
The prevalent facultative anaerobic organisms recovered from mastitis Cows were: S. aureus (15.2%), Str. agalactiae (8.6%), Coryn. pyogenes (7.4%) Str. pyogenes (3.7%), &. coli (7.0%), and Str. bovis (3.3%).
 

perfringeus, B. fragilis, F. aecropharoum, anaerobes, and A. viscous were x present in mastic buffaloes either in pure culture or mixed with other facultative anaerobes with an overall incidence reached 19.0%.

 
The most prevalent facultative anaerobic isolates obtained from mastitic buffaloes: S. aureus, A pyogenes, Str. agalactiae, E. coli, KI. paeumoniae and Str. lactis in order of their frequencies.
 
A total of 43 strains of C. perfringens were recovered from mastitic cows and buffaloes. Out of these, 81.4% were toxigenic and the remaining 16.8% were non toxigenic. Among cows, strains, 96.3% were belonging to type A and 3.7% were of type D. Meanwhile, all toxigenic C. perfringens isolated from buffaloes were belonging to type A only.
In vitro sensitivity of the prevalent obligatory anaerobic isolates to various chemotherapeutic agents was discussed in details.

Main Subjects