BIOCHEMICAL DISORDERS IN SERUM OF TILAPIA NILOTICA EXPOSED TO DIAZINON FOR MEDIUM TERM

Document Type : Original Article

Authors

1 Depts. Of Biochemistry, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt

2 Dept. Mammalian Toxicology, Central Agricultural, Pesticides Laboratory, Egypt.

3 Depts. Of Physiology, Faculty of Veterinary Medicine, Cairo University, Giza 12211

Abstract

Diazinon is an organophosphorus pesticide that Commonly used in eradication of insects. Fish is One of the non-target organisms that may be exposed to diazinon toxicity. In this investigation We studied the effect of exposure of Tilapia nilotica to diazinon at concentrations of 40, 80 and 160 PPM for one, two and four weeks. Lipids profile, Proteins content, proteins fingerprint, bilirubin, Thyroid hormones and certain enzymes activity (GST, CK and AchE) of treated fish serum were Determined. The same parameters were followed Also after a period of ten days of recovery. Dramatic changes were found in most of the measured parameters at all tested concentrations and Times of exposure. Serum protein fractionation by SDS-PAGE revealed disappearance of certain Protein fractions at the different concentrations of Diazinon. Some of these fractions were reappeared again after the recovery period indicating The depressing effect of diazinon on the expression of certain genes.

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Veterinary Medical Journal (Giza)
Volume 49, Issue 1
January 2001
Pages 131-146

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