COMPARISON OF DNA PROBE TECHNIQUE AND C-ELISA FOR DETECTION OF ANAPLASMA MARGINALE IN CATTLE

Document Type : Original Article

Authors

Department of Microbiology and Immunology Faculty of Veterinary Medicine, Cairo University

Abstract

Through this study, recent and modern diagnostic Techniques have been used for diagnosis of cattle Anaplasmosis. Out of 255 cattle that were represented by 162 foreign and 93 native breeds, blood Samples were collected for separation of serum And DNA extraction using very simple and rapid Guanidine thiocyanate and celite method. This investigation, throws a spot light on, the application Of C-ELISA and nucleic acid hybridization using DNA labelled probe for identification of cattle Persistently infected with A. marginale. The naive MSP-5 is one of the outer membrane proteins (19 kDa) of A. marginale that was recognized by Mab and immue sera in C-ELISA. R-MSP-5 was Used as coating antigen and the format of C- ELISA was improved for detection of antibodies Against A. marginale in both acute and carrier anImals. The results of C-ELISA obtained through This work showed that 15.3% of the examined cattle were positive and also identified as 16.7% and 12.9% positive reactivity in foreign and native Breeds respectively. Non-radioactive msp-2 labeled probe was used in dot-blot hybridization to Diagnose bovine anaplasmosis. Only 20% of the Examined animals showed positive signals in dot Blot hybridization, double C-ELISA/dot-blot hybridization positivity was 34 cases proved that these animals were in acute and active stage of infection however, 17 animals showed positive reactivity only with dot blot hybridization. This Work is considered as the first trial for diagnosis of bovine anaplasmosis in Egypt using these recent and modern diagnostic techniques.

Main Subjects

Veterinary Medical Journal (Giza)
Volume 49, Issue 1
January 2001
Pages 45-57

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