COMPARATIVE USE OF AMMONIUM SULPHATE FOR PRECIPTTATION OF COLOSTRAL IMMUNOGLOBULINS

Document Type : Original Article

Authors

1 Departments of Vet. Parasitology , University of Agriculture, Faisalabad

2 Departments of Vet. Microbiology, University of Agriculture, Faisalabad

Abstract

Immunoglobulins (Igs) from the colostrum were successfully precipitated with fertilizer grade ammonium sulphate (NFC)* and the contents were compared with the Igs precipitated by reagent grade ammonium sulphate (E. Merk). There was no significant difference in total Igs concentration precipitated by cither grade ammonium sulphate. Among 10 samples processed, the Igs concentration purified by reagent grade and fertilizer grade ammonium sulphate ranged from 11.75 to 50.00 (20.69 = 3.88) and 10.50 to 49.75 (20.47 + 3.87) gms per liter, respectively. The results revealed that the reagent grade ammonium sulphate can be replaced with fertilizer grade ammonium sulphate.

Main Subjects